Physiological Effects of Rapidus-50 On Chlorophyll, Protein and Lipid Content in Wolffia sp.: Implications for Aquatic Plant Health

Abstract

This study aimed to investigate the physiological effects of varying concentrations of Rapidus-50, a diclofenac-based non-steroidal anti-inflammatory drug (NSAID), on Wolffia sp. Over a five-day exposure period, chlorophyll and lipid contents were measured in treatment groups receiving 10 µL, 100 µL, 1000 µL, and 10000 µL of Rapidus-50. The results demonstrated a clear concentration-dependent decrease in both chlorophyll and lipid levels, with the most pronounced reductions observed at higher concentrations. Statistical analysis using ANOVA confirmed the significance of these changes (p < 0.05), suggesting that the chemical exposure led to photosynthetic disruption and impaired lipid biosynthesis. These findings highlight the ecological risks posed by pharmaceutical pollutants in aquatic ecosystems.