Evaluation Of Haematological Effect and Testosterone Levels in Rattus Norvegicus Administered Pausinystalia Yohimbe

Abstract

This study focused on the effects of Pausinystalia yohimbe ethanolic extract on the hematological parameters and testosterone levels in Wistar rats. Forty-two rats were grouped into six groups (A–F) of seven animals each (4 males and 3 females) and were administered P. yohimbe extract at doses of 25 mg/kg, 50 mg/kg, 75 mg/kg, and 100 mg/kg, with a positive control group receiving Sildenafil citrate at 5 mg/kg and a negative control group receiving distilled water for 14 days. The rats were sacrificed, and 5 ml of blood was collected for hematology tests. There was an increase in hematological parameters (PCV, WBC, hemoglobin, and lymphocytes) as the concentration of P. yohimbe extract increased, except for RBC and platelet levels which decreased. RBC levels decreased from (8.80 ± 0.12) at 25 mg/kg to (8.52 ± 0.02) at 100 mg/kg compared to the control (8.70 ± 0.06). Platelet levels decreased from (764.00 ± 0.58) at 25 mg/kg to (5.70 ± 1.73) at 100 mg/kg compared to the control (532.00 ± 1.15). There was a significant increase in testosterone levels with increasing concentrations of the extract: 25 mg/kg (8.52 ± 0.02) and 50 mg/kg (6.70 ± 0.06), but a decrease at 100 mg/kg (5.40 ± 0.12) compared to the control (3.00 ± 0.12). The study reveals that P. yohimbe ethanolic extracts have a significant effect on the hematological parameters and testosterone levels of Wistar rats.