Evaluation of Antimicrobial and Antioxidant Activity of β-Sitosterol-3-O-Glucoside Isolated from Lannea Kerstingii Engl. & K. Krause (Anacardiaceae).

Authors

  • Njinga, N.S. University of Ilorin
  • Sule, M.I. Ahmadu Bello University, Zaria
  • Pateh, U.U. Ahmadu Bello University, Zaria
  • Hassan, H.S. Ahmadu Bello University, Zaria
  • Magaji, M.G. Ahmadu Bello University, Zaria
  • Abdullahi, S.T. University of Ilorin
  • Bakare-Odunola, T.M. University of Ilorin
  • Bawa, B. National Agency for Food and Drugs Administration and Control (NAFDAC), Nigeria
  • Egharevba, G.O. University of Ilorin
  • Shittu, A.O. University of Ilorin

Keywords:

β-sitosterol-3-O-glucoside, Lannea kerstingii, liquid chromatography, antioxidant, antimicrobial

Abstract

β-sitosterol-3-O-glucoside was isolated using dry vacuum liquid chromatography. It was characterized using 1H-NMR, 13C-NMR and 2D NMR spectra. The antimicrobial activity was determined using agar diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were determined using broth dilution method. Antioxidant activity was determined through reaction of compound with DPPH radical. The compound was active against S. aureus, Methicillin Resistant Staphylococcus aureus, P. mirabilis, S. typhi, K. pneumoniae, E. coli, B. subtilis, C. albicans and C. tropicalis with zone of inhibition ranging from 22.0 ± 0.4 to 34.0 ± 0.3 mm and inactive against P. aeroginosa, Proteus vulgaris and C. krusei. The MIC ranged from 25.0 to 50.0 μg/ml while the MBC/MFC ranged from 50.0 to 200.0 μg/ml. The % DPPH scavenging activity at 4.0x103 µg/ml was found to be 81.44 ± 0.41 %. This result shows the high antioxidant and wide spectrum antimicrobial activity of β-sitosterol-3-O-glucoside which can be a potential lead drug for resistant bacteria such as MRSA, and a potential source of natural antioxidants.

Author Biographies

Njinga, N.S., University of Ilorin

Department of Pharmaceutical and Medicinal Chemistry, University of Ilorin, Ilorin, Nigeria

Sule, M.I., Ahmadu Bello University, Zaria

Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria,
Nigeria

Pateh, U.U., Ahmadu Bello University, Zaria

Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria,
Nigeria

Hassan, H.S., Ahmadu Bello University, Zaria

Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria,
Nigeria

Magaji, M.G., Ahmadu Bello University, Zaria

Department of Pharmacology and therapeutics, Ahmadu Bello University, Zaria, Nigeria

Abdullahi, S.T., University of Ilorin

Department of Pharmaceutical and Medicinal Chemistry, University of Ilorin, Ilorin, Nigeria

Bakare-Odunola, T.M., University of Ilorin

Department of Pharmaceutical and Medicinal Chemistry, University of Ilorin, Ilorin, Nigeria

Bawa, B., National Agency for Food and Drugs Administration and Control (NAFDAC), Nigeria

National Agency for Food and Drugs Administration and Control (NAFDAC), Nigeria

Egharevba, G.O., University of Ilorin

Department of Chemistry, University of Ilorin, Ilorin, Nigeria

Shittu, A.O., University of Ilorin

Department of Pharmaceutics and industrial pharmacy, University of Ilorin, Ilorin, Nigeria

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Published

2016-03-30

How to Cite

Njinga, N.S., Sule, M.I., Pateh, U.U., Hassan, H.S., Magaji, M.G., Abdullahi, S.T., Bakare-Odunola, T.M., Bawa, B., Egharevba, G.O., & Shittu, A.O. (2016). Evaluation of Antimicrobial and Antioxidant Activity of β-Sitosterol-3-O-Glucoside Isolated from Lannea Kerstingii Engl. & K. Krause (Anacardiaceae). International Journal of Science for Global Sustainability, 2(1), 9. Retrieved from https://fugus-ijsgs.com.ng/index.php/ijsgs/article/view/281

Issue

Vol. 2 No. 1 (2016): INTERNATIONAL JOURNAL OF SCIENCE FOR GLOBAL SUSTAINABILITY (IJSGS)

Section

Articles